What is the function of Bisacrylamide in sds page?

Bisacrylamide is used as a cross-linking agent in the preparation of polyacrylamide gels for SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis). It facilitates the formation of a three-dimensional gel matrix by linking acrylamide molecules together, which helps to create a porous structure that allows for the separation of proteins based on their size. The concentration of bisacrylamide influences the gel's pore size, thus affecting the resolution and separation efficiency of the proteins during electrophoresis.