How do you get the 2nd lot 192 remedy in BioShock?

To acquire the 2nd Lot 192 remedy in BiOShock, you need to first reach the "Fort Frolic" area. Once there, locate the "Theater" where you will find a series of events leading to the quest for Lot 192. You'll need to defeat the character called "Sander Cohen" and complete his tasks to eventually gain access to the remedy. After that, head to the "Medical Pavilion" and find the Lot 192 in a nearby storage room.