In your simulated two factor cross why might your actual experimental values have been different from the values you expected?

Actual values might differ from expected values in a two-factor cross due to genetic linkage, where two loci are located close together on the same chromosome and do not assort independently. Another reason could be the influence of environmental factors on the expression of traits. Lastly, errors in data collection or analysis could also lead to discrepancies between actual and expected values.