In which of the famous Lincoln-Douglas debates did Lincoln respond to Douglas' popular sovereignty stand with an argument of his own?

In the second Lincoln-Douglas debate, held in Freeport, Illinois, on August 27, 1858, Abraham Lincoln directly challenged Stephen A. Douglas's stance on popular sovereignty. Lincoln posed the "Freeport Question," asking whether the people of a territory could exclude slavery before becoming a state. Douglas's response, which suggested that local legislation could effectively prevent slavery, ultimately alienated him from southern Democrats and highlighted the contentious issue of slavery's expansion.