What are the key differences between HPLC normal phase and reverse phase chromatography techniques?

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1118196

2026-08-20 14:40

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In normal phase HPLC, the stationary phase is polar and the mobile phase is nonpolar, while in reverse phase HPLC, the stationary phase is nonpolar and the mobile phase is polar. This difference in polarity affects how compounds interact with the stationary phase, leading to different separation mechanisms and selectivity in each technique.

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