The Treponema pallidum hemagglutination assay (TPHA) is primarily used to detect syphilis, typically transmitted through sexual contact. However, it can also yield positive results in cases of non-sexual transmission, such as from mother to child during pregnancy (congenital syphilis) or through blood transfusions, although the latter is rare due to strict screening processes. Additionally, certain autoimmune diseases and infections can cause cross-reactivity, leading to false positives in the test.
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