How can you distinguish between male and female kittens?

One way to distinguish between male and female kittens is by looking at their genitalia. Male kittens have a larger distance between the anus and the genital opening, while female kittens have a shorter distance. Additionally, male kittens have a small bump called a scrotum, while female kittens do not. It is best to consult a veterinarian for accurate identification.