Were there any females in the Pony Express?

Yes, there were females associated with the Pony Express, though they were not officially part of the rider ranks. Some women took on roles such as cooks, laundresses, and in other support capacities. One notable figure is Buffalo Bill's sister, who is said to have ridden the route disguised as a male rider. Overall, while female riders were rare, women played essential roles in supporting the operation.