Fluid used on autoclave to sterilize instruments?

The fluid used in an autoclave to sterilize instruments is typically distilled water, which generates steam when heated. In some cases, a specific sterilization solution may be added to enhance the process, but pure water is usually sufficient for effective steam sterilization. The autoclave operates by using high-pressure steam at temperatures usually around 121°C (250°F) or higher to kill bacteria, viruses, and spores on the instruments.