What happen if you gorget to add iodine in gram satin negative?

If iodine is omitted during the Gram staining process, the crystal violet dye will not form a stable complex with the Gram-positive bacteria, which typically requires iodine to fix the dye. As a result, both Gram-positive and Gram-negative bacteria may appear pink after the decolorization step, leading to misinterpretation of the results. This could hinder proper identification and treatment of bacterial infections. Therefore, iodine is crucial for accurate Gram staining.