The Compromise of 1850 which endorsed the idea of popular sovereignty essentially disregarded which previous piece of legislation?

The Compromise of 1850 effectively disregarded the Missouri Compromise of 1820, which had established a line dividing free and slave states. By endorsing popular sovereignty, the Compromise allowed new territories to decide for themselves whether to permit slavery, undermining the earlier agreement that had sought to maintain a balance between free and slave states. This shift contributed to increased tensions over slavery in the United States.