In ASP.NET 3.5, session variables can be utilized to store user-specific data across multiple pages within a web application. You can access the Session object through Session["VariableName"] to set or retrieve values. For example, to store a username, you would use Session["Username"] = "JohnDoe";, and to retrieve it, you would use string username = (string)Session["Username"];. Ensure to manage session state properly to avoid excessive memory usage and to handle session expiration appropriately.
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