What groups of her time opposed Mary queen of Scots?

Mary Queen of Scots faced opposition from various groups during her reign, notably Protestant factions in Scotland who viewed her Catholic faith as a threat to the Protestant Reformation. Additionally, the Scottish nobility, particularly the powerful Lords of the Congregation, sought to limit her authority and advance their own interests. English Protestants, including Queen Elizabeth I, also opposed her, fearing her claim to the English throne due to her Catholic lineage. These combined pressures ultimately led to her downfall and imprisonment.