In the light-independent reactions, also known as the Calvin cycle, carbon dioxide molecules are fixed into organic molecules through a series of enzymatic reactions. Specifically, CO2 is combined with ribulose bisphosphate (RuBP) to form 3-phosphoglycerate (3-PGA) using the enzyme RuBisCO. This process ultimately leads to the synthesis of glucose and other carbohydrates, which serve as energy sources for the plant. The reactions occur in the stroma of chloroplasts and do not directly require light, but they rely on the ATP and NADPH produced during the light-dependent reactions.
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