How much would a tune up cost for a lamborghini?

If we are talking spark plugs and a quick electronics check for a fairly current car, $1,000 to $3,000 depending on model.

If we are tuning to fix a problem: if you need to know the cost, don't buy one.

For older ones, parts a scarce and difficult to get. The carburetors are notorious for requiring a ton of work often. Look for a big bill.