In what ways did regional interests assert themselves after the creation of the US?

After the creation of the United States, regional interests asserted themselves primarily through economic, political, and social differences. The North and South developed distinct economies, with the North focusing on industrialization and the South relying on agriculture and slavery, leading to tensions over tariffs and trade policies. Additionally, states' rights advocates in various regions pushed back against federal authority, exemplified by events like the Nullification Crisis. These regional disparities ultimately contributed to sectional conflicts that paved the way for the Civil War.