What do the only difference among the four DNA nucleotides?

The only difference among the four DNA nucleotides lies in their nitrogenous bases. The four nucleotides are adenine (A), thymine (T), cytosine (C), and guanine (G). Each base pairs specifically (A with T and C with G) to form the rungs of the DNA double helix, contributing to the genetic code. The sugar and phosphate backbone remains consistent across all four nucleotides, emphasizing the significance of the nitrogenous bases in determining genetic information.