What is skunk spray made of and how does it work as a defense mechanism for skunks?

Skunk spray is made of chemicals called thiols, which are sulfur-containing compounds. When a skunk feels threatened, it can release this spray from glands near its anus. The strong odor of the spray is very unpleasant to predators and can cause irritation to their eyes and nose, deterring them from attacking the skunk. This defense mechanism helps skunks protect themselves from potential threats.