Sasuke Uchiha first used his Mangekyō Sharingan during the battle against Danzo Shimura in "Naruto: Shippuden." He activated it to employ his powerful techniques, including Amaterasu and later Susanoo, showcasing his growth and mastery of the eye's abilities. This moment was significant as it highlighted Sasuke's emotional turmoil and desire for revenge against Danzo for his actions.
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