What is a sense probe in insitu hybridisation?

A sense probe in in situ hybridization is a nucleotide sequence that is complementary to the target RNA or DNA sequence of interest, allowing for the detection of specific genes within tissue samples. Unlike antisense probes, which bind to the target sequence to visualize gene expression, sense probes bind to the non-template strand and serve as a control to confirm the specificity of the hybridization signal. The presence of a signal with a sense probe typically indicates non-specific binding, while a lack of signal supports the specificity of the antisense probe results.