Magnification and resolution is in light microscope is about 500x and 0.2micro metres respectively than human eye.In e.microscope magnification is about 200,000x and resolution is 0.0005micro metres. You cannot observe live specimen through electron microscope. Lenses in light microscope are glass while e.microscope use magnetic lenses. E.microscope is relatively very larger than light microscope. You need special knowledge to handle electron microscope. You can observe through naked eye in e.microscope. A beam of electrons is sent through specimen in e.microscope.In light microscope a beam of light is sent. Electron microscope is very complex relative to light microscope.
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