What is the correct sequences of events in the production of recombinant DNA?

The production of recombinant DNA involves several key steps: First, a specific gene of interest is isolated using restriction enzymes that cut DNA at particular sequences. Next, this gene is inserted into a plasmid vector, which also contains a marker gene for selection. The recombinant plasmid is then introduced into a host organism, often bacteria, through a process called transformation. Finally, the host cells replicate the plasmid, producing multiple copies of the recombinant DNA and expressing the inserted gene.