What are the inputs and outputs of Preparatory Reaction?

The Preparatory Reaction, also known as the link reaction or pyruvate decarboxylation, occurs in the mitochondria after glycolysis. The primary inputs are pyruvate (produced from glycolysis), NAD⁺, and coenzyme A (CoA). The outputs of this reaction are acetyl-CoA, carbon dioxide (CO₂), and NADH. This reaction serves as a crucial link between glycolysis and the Krebs cycle, facilitating the conversion of glucose-derived pyruvate into a form that can enter the Krebs cycle.