In a list of items you do not put a comma. However if you use it as a coordinating conjunction you do.
Example: I ate Oranges, Apples, and bananas this week.
Yo comí naranjas, manzanas y bananas esta semana.
Martha carried all the light packages, and Sam carried all the heavy ones.
Martha cargo todos los paquetes livianos, y Sam cargo todo los pesados.
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