What would be the effect of using FTM tubes with broad pink zone?

Using FTM (Fluid Thioglycollate Medium) tubes with a broad pink zone indicates the presence of a significant amount of dissolved oxygen, suggesting that the medium is not effectively reducing oxygen levels. This can lead to the growth of aerobic bacteria, which may mask the growth of anaerobic bacteria that would typically thrive in a more reduced environment. Consequently, the results of microbial cultures may be skewed, potentially leading to false interpretations regarding the sample's microbial composition. Properly maintaining anaerobic conditions is crucial for accurate microbiological analysis.