Do a female and male retic who have been housed together for 2 years now since they were babies have to be separated?

Yes, a female and male reticulated python (retic) that have been housed together for two years should be separated, especially if they are nearing sexual maturity. Housing them together can lead to stress, territorial disputes, or potential breeding, which can pose health risks to both snakes. It's important to provide them with individual habitats to ensure their well-being and safety.