How would set up an experiment testing the effectiveness of a vaccine on 100 rats?

To test the effectiveness of a vaccine on 100 rats, I would randomly divide the rats into two groups: 50 vaccinated and 50 control (unvaccinated). The vaccinated group would receive the vaccine, while the control group would receive a placebo. After a defined period, both groups would be exposed to the virus or pathogen the vaccine targets, and I would monitor and compare the health outcomes, immune response, and disease incidence in both groups to assess the vaccine's effectiveness. Ethical considerations, including humane treatment and minimizing suffering, would be prioritized throughout the experiment.