Was Queen Elizabeth 1 locked in the Tower of London?

No, Queen Elizabeth I was never locked in the Tower of London. However, she was imprisoned there for a brief period in 1554 when she was accused of complicity in a plot to overthrow her half-sister, Queen Mary I. After her release, she went on to rule England from 1558 until her death in 1603, becoming one of the most famous monarchs in history.