How do you calculate Resolution between two Peaks?

Resolution between two peaks in chromatography is calculated using the formula:

[ R = \frac{2(t_R2 - t_R1)}{W_1 + W_2} ]

where ( t_R2 ) and ( t_R1 ) are the retention times of the two peaks, and ( W_1 ) and ( W_2 ) are the widths (at baseline) of the respective peaks. A higher resolution value indicates better separation between the peaks, with values greater than 1.5 typically considered acceptable for good separation.