Why you re using peg in hybridoma?

PEG (polyethylene glycol) is used in hybridoma technology primarily to promote the fusion of myeloma cells and antibody-producing B cells. It facilitates the merging of cell membranes, leading to the creation of hybrid cells that can produce specific monoclonal antibodies. Additionally, PEG helps to increase the yield of viable hybrid cells by promoting cell viability during the fusion process. Overall, its use enhances the efficiency and success rate of generating hybridomas.