Isolation often leads to speciation, because as each isolated population evolves new characteristics, the separate populations eventually get DNA that is too different for the two to breed and have fertile offspring (this is the point when speciation has occurred). In the case that there is not isolation, the whole species must slowly evolve until it becomes a new species. However, here the line between where the speciation actually occurred becomes blurry, because it doesn't happen in a single generation.
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