In the movie osmosis jomes what are examples of the first ane second lines of defense working to protect frank from pathogens?

In "Osmosis Jones," the first line of defense is represented by the physical barriers, such as the skin and mucus membranes, which protect Frank from pathogens entering his body. The second line of defense is exemplified by the immune response initiated by white blood cells, like Thrax's attempts to invade, prompting the action of immune cells like Osmosis Jones and Drix, who work to eliminate the invading pathogens and protect Frank's health. Together, these defenses illustrate the body's layered approach to preventing and combating infections.