The broth in the flask became cloudy and contaminated due to the presence of microorganisms, which likely entered the flask through exposure to air or improper sterilization. The nutrient-rich environment of the broth allowed these microbes to multiply rapidly, leading to turbidity and visible signs of contamination. In contrast, flasks that were sealed or properly sterilized remained clear because they were protected from microbial invasion. This demonstrates the importance of aseptic techniques in microbiological experiments.
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