King James I persecuted Puritan Separatists primarily because he viewed their desire to separate from the Church of England as a threat to the unity and authority of his reign. He believed that religious uniformity was essential for political stability and saw the Separatists' dissenting beliefs as a challenge to both his power and the established church. Additionally, the king was concerned that such movements could inspire broader dissent and undermine his absolute rule. This led to increased persecution, including arrests and forced conformity to Anglican practices.
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