Does the number of mitochondria increase during interphase?

Yes, the number of mitochondria can increase during interphase. During this phase of the cell cycle, particularly in the G1 and S phases, cells prepare for division by growing and duplicating their organelles, including mitochondria. This increase supports the higher energy demands of the cell as it prepares for mitosis. Mitochondrial biogenesis is regulated by various factors, including cellular energy needs and signaling pathways.