What are the advantages and disadvantages of Bradford protein assay?

1 answer

Answer

1138435

2026-04-30 10:26

+ Follow

Advantages:

Compatible with most buffers, reagents and preparations. One step procedure that is sensitive and accurate.

Disadvantages:

Non linear correlation over large range of protein concentrations. The dye binds to surface of cuvettes so calibration curves need to be made every time. Also the dye binds to test tubes so washing after use immediate use is a must. High concentration detergents can interfere with assay.

ReportLike(0ShareFavorite

Copyright © 2026 eLLeNow.com All Rights Reserved.