How is clostridium perferingens detected?

C. perfringens can be diagnosed by Nagler's Reaction where the organism is cultured on an egg yolk media plate. One side of the plate contains anti-alpha-toxin, while the other side does not. The suspect organism is placed on both sides. An area of turbidity will form around the side that does not have the anti-alpha-toxin,indicating that this is C. perfringens.