What would have happened if we had cut both the jellyfish glo gene and the puc18 plasmid with the EcoR1 restriction enzyme?

If both the jellyfish glo gene and the puc18 plasmid were cut with the EcoRI restriction enzyme, compatible sticky ends would be generated on both DNA fragments. This would allow the jellyfish glo gene to be inserted into the puc18 plasmid through a process called ligation. As a result, the plasmid could be used to clone the glo gene, facilitating its expression in a host organism for further study or application. This technique is a fundamental method in genetic engineering and molecular Biology.