What is the principle of the total hemolytic complement kit?

The principle of the total hemolytic complement kit is based on the ability of complement proteins in serum to lyse antibody-sensitized red blood cells. When the serum is mixed with these sensitized cells, the complement system is activated, leading to the formation of the membrane attack complex that disrupts the cell membrane, resulting in hemolysis. The extent of hemolysis can be quantitatively measured, providing an indication of the total complement activity present in the serum sample. This test is useful for evaluating the complement system's functionality in various immunological and pathological conditions.