How do you prepare positive charged slide for immunohistochemestry?

To prepare positively charged slides for immunohistochemistry, first, ensure that the slides are clean and free of any contaminants. Next, pre-treat the slides by rinsing them in a solution of 0.1 M phosphate-buffered saline (PBS) and then drying them. Following this, the slides can be coated with a positively charged substrate or treated with a positively charged solution, such as poly-L-lysine, to enhance tissue adhesion. Finally, the slides can be stored in a moisture-free environment until ready for use.