To prepare Hager's reagent for alkaloid testing, dissolve 1 gram of mercuric chloride (HgCl₂) in 100 mL of distilled water. This solution can be used to precipitate alkaloids in plant extracts, forming a yellow precipitate that indicates the presence of alkaloids. Ensure to handle mercuric chloride with care, as it is toxic and hazardous. Store the prepared reagent in a dark glass bottle to protect it from light.
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