Why is the x100 objective in a microscope not often used?

The x100 objective in a microscope is not often used because it requires a very thin specimen and optimal slide preparation to avoid distortion and artifacts. Additionally, this objective typically necessitates the use of immersion oil to minimize light refraction, which can complicate the setup and handling. The increased magnification also means that even slight vibrations or movements can significantly affect the image quality, making it less practical for routine observations.