Why is column revers phase in hplc?

Column reverse phase in High-Performance Liquid Chromatography (HPLC) refers to the use of a nonpolar stationary phase and a polar mobile phase. This configuration allows for the separation of compounds based on their hydrophobicity, where more hydrophobic compounds interact more strongly with the stationary phase and elute later. Reverse phase HPLC is widely used because it provides high resolution and sensitivity for a variety of analytes, making it suitable for pharmaceuticals, environmental samples, and biological fluids.