How many FADH2 are produced during forming acetyl CoA?

During the conversion of pyruvate to acetyl CoA, which occurs in the mitochondria, one molecule of FADH2 is not produced. Instead, the process generates one molecule of NADH for each pyruvate molecule converted. Since one glucose molecule produces two pyruvate molecules, a total of two NADH molecules are produced during the formation of acetyl CoA from one glucose molecule. Thus, no FADH2 is produced in this specific step.