What is the protocol for performing a DTT reduction step in a SDS-PAGE experiment?

The protocol for performing a DTT reduction step in a SDS-PAGE experiment involves adding DTT (dithiothreitol) to the protein sample to break disulfide bonds, heating the sample to denature the proteins, and then running the sample on a gel to separate the proteins based on size. This step helps to ensure accurate protein analysis by reducing disulfide bonds that can affect protein migration on the gel.