Is crystal violet positive or negative staining?

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1285553

2026-07-23 02:00

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Crystal violet is a basic dye used predominantly in positive staining techniques. It binds to negatively charged components on the surface of cells, such as nucleic acids and proteins, which results in the cells appearing purple under the microscope. This is particularly useful in differentiating bacteria during Gram staining, where Gram-positive bacteria retain the crystal violet dye, while Gram-negative bacteria do not.

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