How does the exonuclease capability of DNA Pol I and Pol III differ?

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1214038

2026-08-20 14:00

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DNA Polymerase I has both 5' to 3' exonuclease activity and 3' to 5' proofreading exonuclease activity, allowing it to remove RNA primers and correct errors during DNA synthesis. In contrast, DNA Polymerase III primarily possesses 3' to 5' exonuclease activity for proofreading, ensuring high fidelity during DNA replication. While both enzymes are involved in DNA replication, their exonuclease functionalities serve different roles: Pol I for primer removal and Pol III for error correction during synthesis.

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