Can I spray enamel paint over the top of acrylic paint on a car?

Well, honey, technically you can spray enamel paint over acrylic paint on a car, but it's not the ideal scenario. The two have different properties, so the enamel might not adhere properly and could lead to chipping or peeling. If you want a paint job that lasts, I'd recommend sanding down the acrylic paint before applying the enamel.