What is the limitation of DNA polymerase in replication?

DNA polymerase has a limitation in that it can only synthesize new DNA strands in the 5' to 3' direction. This means it cannot initiate synthesis on its own and requires a primer to provide a free 3' hydroxyl group. Additionally, DNA polymerase cannot remove RNA primers or repair mistakes without the assistance of other enzymes, which can complicate the replication process and lead to potential errors in the DNA sequence.