What two of the genes are most likely to be separated during crossover?

Genes that are located far apart on a chromosome are more likely to be separated during crossover. This is because the probability of crossover occurring between two genes increases with the distance between them, as there is a greater chance that a crossover event will occur in that region during meiOSis. Therefore, genes that are on opposite ends of a chromosome are the most likely to be separated during crossover.